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Ungakhetha Kanjani Izitsha Ezilungile ZeSiko Lamaseli?

2024-11-29

Ukukhetha i-cultureware elungile kubalulekile ekuqinisekiseni ukukhula kweseli okuphelele kanye nemiphumela yokuhlola. Lapho ukhetha imikhumbi ye-cell culture, kubalulekile ukucabangela izici ezifana nohlobo lweseli, inhloso ethize yesiko lakho, isikali sesiko, uhlobo lwe-culture medium, izinto zokwakha kanye nosayizi wemikhumbi, ukwelashwa kwendawo, izivalo ezifanele. ukushintshana kwegesi, nokuhambisana kwazo nemishini yakho yaselabhorethri.

Nazi izici ezibalulekile okufanele uzicabangele lapho ukhetha umkhumbi ofanele wamaseli

1. Khetha isitsha se-cell culture ngokusekelwe ohlotsheni lwamaseli

Amaseli Anamathelayo

Lawa maseli adinga indawo ukuze axhumeke futhi asabalale. Kumaseli abambelelayo, uzodinga imikhumbi enendawo engaphezulu enikeza isikhala esanele sokunamathiselwa kweseli nokukhula.

Izibonelo Amaflaski alashwe ngezicubu, izitsha ze-petri, namapuleti anemithombo eminingi.

Amaseli Amisiwe

Lawa maseli akhula entanta phakathi nendawo, ngakho indawo engaphezulu ayibhekwa.

IziboneloAmaflaski amasiko ezicubu, amaflaski esipina, noma ama-bioreactors wamasiko amakhulu okumiswa.

2. Khetha umkhumbi we-cell culture ngokusekelwe kusayizi (Ivolumu Yamandla)

Amasiko Asezingeni Elincane

Ngokuhlolwa kwezinga elincane noma ukuhlolwa okuphezulu, imikhumbi emincane ilungile.

Izibonelo Amapuleti emithombo eminingi (6, 24, 96-cell culture plates),Petri izitsha, noma amaflaski e-T25.

Amasiko Amakhulu

Uma udinga ukukhulisa inani elikhulu lamaseli, imikhumbi emikhulu noma ama-bioreactors angcono.

Izibonelo T75 kanye T175 cell culture flasks, bioreactors, noma spinner flasks for suspension cell cultures.

3. Khetha isitsha se-cell culture ngokusekelwe ekwelapheni okungaphezulu

I-Tissue Culture-Treated Surfaces

Imikhumbi iphathwa kusengaphambili ukuze ikhuthaze ukunamathiselwa kweseli, okuyenza ifanelekele izinhlobo zamaseli anamathelayo. Lezi ngokuvamile zimbozwe ngezinto ezifana ne-collagen, i-fibronectin, noma ezinye izingxenye ze-matrix ezingaphandle kwamaselula.

Izindawo Ezingalashwa

Ilungele amasiko amisiwe noma lapho amaseli engadingi ukunamathela phezulu. Ngokuvamile zisetshenziselwa amaseli akhula ngokukhululeka phakathi nendawo.

4. Khetha isitsha se-cell culture esekelwe ezintweni

I-polystyrene evame ukusetshenziselwa izicelo ezijwayelekile zesiko lamaseli. Icacile, ivumela ukuhlolwa okubonakalayo okulula, futhi isebenza kahle kuwo womabili amaseli anamathelayo nawamisiwe.

I-Polycarbonate noma i-Polypropylene isetshenziselwa izinhlelo ezithile ze-bioreactor kanye nemikhumbi edinga ukuguquguquka okwengeziwe noma ukwelashwa okuqondile kwendawo.

Ingilazi esetshenziselwa isiko lezicubu ezijwayelekile ngenxa yezindleko nokuphuka, imikhumbi yengilazi ingase ifanelekele ukusetshenziswa okuthile noma amasiko amakhulu.

5. Khetha iizinto ezidlekayo zesiko lamaselingokusekelwe ekwakhiweni komkhumbi

Amaflaski

Ngokwesiko elijwayelekile lamaseli, ama-T-flasks (T25, T75, T150) avame ukusetshenziswa. Indawo eyisicaba inikeza indawo enhle yokunamathiselwa kweseli nokukhula. Angasetshenziselwa kokubili amaseli abambelelayo kanye namasiko amisiwe uma izimo ezifanele zigcinwa.

Petri Izitsha

Kujwayelekile kumasiko wezinga elincane kanye nokuhlolwa okudinga ukubhekwa, okufana nokuhlolwa kokwakheka kwekoloni.

Amapuleti Ama-Multi-Well

Lokhu kuwusizo ekuhlolweni komphumela ophezulu kanye nokuhlolwa kwezinga elincane. Amapuleti ana-6, 12, 24, 48,96, noma imithombo engu-384 iyatholakala, futhi ilungele ukuhlolwa okusekelwe kumaseli, ukukhishwa kwe-cytokine, ukuhlolwa kwezidakamizwa, nezinye izinhlelo zokusebenza eziphuma phambili.

Ama-Spinner Flasks

Isetshenziselwa amasiko amaseli amisiwe, ikakhulukazi emiqulu emikhulu lapho ukuyaluza okulawulwayo kudingekile ukuze kugcinwe ukukhula kwamaseli nokugwema ukunqwabelana kwamaseli.

Ama-Bioreactors

Kusiko lokumiswa kwesilinganiso esikhulu, ama-bioreactors avumela ukulawula okuyinkimbinkimbi ngaphezulu kwezimo zemvelo (isb., i-pH, izinga lokushisa, i-oxygenation) futhi asetshenziselwa ukukhiqizwa kwevolumu enkulu, njengokukhiqiza i-biopharmaceutical.

6. Khetha isitsha se-cell culture esisekelwe kubunyumba kanye nokukhipha umoya

Ubunyumba

Qinisekisa ukuthi umkhumbi awunamagciwane noma uvalwe amagciwane ukuze ugweme ukungcoliswa. Imikhumbi eminingi yesiko lokuhweba ifakwe inzalo ngaphambili, kodwa hlala uhlola ukupakishwa.

Ukungenisa umoya

Eminye imikhumbi, njengamaflask, iza namakepisi aphuma umoya noma izihlungi ukuvumela ukushintshaniswa komoya kuyilapho ivimbela ukungcola. Lokhu kubalulekile lapho kukhiqizwa amaseli ezimeni eziphakeme kakhulu.

7. Khetha isitsha se-cell culture ngokusekelwe ekusebenziseni kalula

I-Autoclavable vs. Okulahlwayo

Eminye imikhumbi yamasiko ingenziwa i-autoclaved ukuze iphinde isetshenziswe (isb., amabhodlela engilazi, amaflask athile epulasitiki), kuyilapho eminye isetshenziswa kanye futhi ilahlwa (isb., izitsha zepulasitiki zepetri, amapuleti anemithombo eminingi).

Ukuphatha kanye Nezokuthutha

Cabanga ngokukhululeka kokudlulisa amaseli phakathi kwemikhumbi. Isibonelo, amapuleti anemithombo eminingi angase adinge amapuleti akhethekile ukuze aphathwe kalula ngemishini efana nama-pipettor azenzakalelayo.

8. Khetha isitsha se-cell culture esisekelwe kuvolumu ye-culture medium

Khetha umkhumbi ongakwazi ukumumatha umthamo ofunekayo we-culture medium ngaphandle kokumosha izinsiza. Uma usebenza ngesiko levolumu ephezulu, ama-flasks amakhulu noma ama-bioreactors angase adingeke, kuyilapho amavolumu amancane afanele izitsha ze-cell culture noma amapuleti.

9. Khetha isitsha se-cell culture ngokusekelwe ekucatshangelweni kwezindleko

Okulahlwayo uma kuqhathaniswa nokusebenziseka kabusha

Imikhumbi yepulasitiki elahlwayo iyabiza futhi inciphisa ubungozi bokungcoliswa, kodwa ingabiza emisebenzini emikhulu. Imikhumbi yengilazi esebenziseka kabusha inezindleko eziphezulu zangaphambili kodwa ingafakwa amagciwane futhi iphinde isetshenziswe izikhathi eziningi, okuyenza yonge kakhulu ukuze isetshenziswe isikhathi eside.

Ukuphumelela Kwevolumu

Qinisekisa ukuthi usayizi womkhumbi ufanelekile ukugwema ukumoshakala kwezinto, ikakhulukazi uma usebenzisa imidiya yokukhula ebizayo noma ama-reagents.

10. Khetha isitsha se-cell culture ngokusekelwe ezidingweni ezithile zohlelo lokusebenza

Ukufanekisa

Uma udinga ukubona amaseli ngeso lengqondo ngaphansi kwesibonakhulu, khetha imikhumbi enezinto ezicacile nezilinganiso ezifanele zokusetha kwakho isithombe (isb., amapuleti anemithombo eminingi yokuhlola okuqukethwe okuphezulu noma izitsha ezingaphansi kwengilazi zokuthwebula amaseli bukhoma).

Ukuyaluza okulawulwayo

Kumasiko amaseli amisiwe, cabangela amaflaski esipina noma ama-bioreactors ahlinzeka ngokunyakazisa okulawulwayo ukuze kugcinwe amaseli emisiwe ngokulinganayo.

Isiphetho

Ukukhetha umkhumbi olungile wamaseli kudinga ukulinganisa izici eziningana, okuhlanganisa uhlobo lweseli, isikali samasiko, ukuhambisana kwezinto ezibonakalayo, kanye nezidingo ezithile zokuhlola. Amaseli abambelelayo azodinga izindawo ezikhuthaza ukunamathiselwa, kuyilapho amaseli amisiwe ezuza kumavolumu amakhulu kanye nokuyaluza. Ngomsebenzi omncane, amapuleti anemithombo eminingi noma ama-T-flasks angase anele, kuyilapho amasiko amakhulu angase adinge ama-spinner flasks noma ama-bioreactors. Ngaso sonke isikhathi qinisekisa ukuthi imikhumbi ihlangabezana nezidingo zakho zokungazali nokuphatha, futhi ucabangele ukuphumelela kwezindleko ngokusekelwe ekusebenziseni kwakho.

Ngokucabangela lezi zici, ungakhetha umkhumbi olungile ohlinzeka ngezimo ezifanele zesiko leseli lakho nemigomo yokuhlola.

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