Umhlahlandlela Wamathiphu Ahlukene We-Pipette Yelabhorethri

Yiziphi amathiphu e-pipette?

Amathiphu e-Pipette ayizesekeli ezilahlwayo zamapayipi asetshenziselwa ukudlulisa uketshezi ngokunembile. Ziza ngosayizi abahlukahlukene, izinto zokwakha, kanye nezinhlobo, njengokujwayelekile, ukunamathela okuphansi, amathiphu ahlungiwe, kanye nobude obunwetshiwe.

Amathiphu e-Pipette abalulekile kumalebhu esayensi futhi asetshenziswa kabanzi kuyo yonke isayensi yempilo, ikhemistri, imithi, i-biotechnology, kanye nebhayoloji yamangqamuzana. Ngenxa yezinhlelo zokusebenza ezihlukene, izinhlangano ezilawulayo emhlabeni wonke zibeka izindinganiso zekhwalithi ukuze ziqinisekise ukunemba nokwethembeka ocwaningweni. I-Cotaus, umkhiqizi owaziwayo wezinto ezisetshenziswayo zebhayoloji e-China, ukhiqiza amathiphu e-pipette ekhwalithi ephezulu aqinisekisiwe nge-ISO, CE, kanye ne-FDA, aqinisekisa ukwethembeka nokuhambisana nocwaningo lwesayensi.

Namuhla, ake sihlole izinhlobo ezahlukene zamathiphu we-pipette, ukuze siqonde izici zabo eziyingqayizivele kanye nezinhlelo zokusebenza ekuphatheni okuqondile kwe-liquid.

Izinhlobo ezahlukene zamathiphu we-pipette

1. Amathiphu E-Pipette Ajwayelekile (Universal).

Amathiphu we-pipette ajwayelekile, owaziwa nangokuthi amathiphu wendawo yonke, ngokuvamile enziwe ngekhwalithi ephezulu, i-autoclavable polypropylene. Ziwuhlobo oluvame kakhulu lwesisetshenziswa se-pipette esisetshenziswa kumalabhorethri anezidingo ezahlukahlukene zokusebenza ezisukela ekunembeni okuphezulu kuya ekukhishweni kwe-reagent ngokubekezelela okukhulu, okuklanyelwe ukulingana nezinhlobo eziningi zezinhlobo namamodeli we-pipette, okuwenza aguquguquke futhi afanele uketshezi olujwayelekile. ukuphatha imisebenzi. Itholakala kuzo zombili izinguqulo eziyinyumba nezingezona inyumba kuye ngezidingo ezithile zokuhlolwa.

Amathiphu Angeyona INzalo vs. Inyumba

Amathiphu Angeyona Inyumba:Lezi zingasetshenziselwa izinqubo zelebhu evamile lapho ubunyumba bungabalulekanga. Ayabiza kakhulu emisebenzini evamile noma amasampula angazweli.

Amathiphu Oyinyumba: Abalulekile ezinhlelweni ezibucayi ezifana ne-microbiology, i-molecular biology, nokuhlolwa komtholampilo, njengoba akhishwa inzalo ngaphambili futhi aqinisekisiwe ukuthi awangcolisi njenge-RNase, i-DNase, nama-endotoxins njll. Kungase kubonakale kukhanga i-autoclave amathiphu angewona oyinyumba endaweni oyinyumba. kodwa i-autoclaving ingase iqede ingozi yokungcola okubangelwa izinto eziphilayo, lokhu akusho ukuthi amathiphu ngeke I-RNase ne-DNase.

Uma udinga ukwenza izivivinyo ezizwelayo lapho kudingeka khona lokhu, kufanele ukhethe amathiphu e-pipette oyinyumba kumkhiqizi ongaqinisekisa ukuthi amathiphu akhe awanawo i-RNase ne-DNase.

I-Cotausamathiphu ajwayelekileziza ngosayizi abahlukene bevolumu (isb., 10 µL, 20 µL, 50 µL, 100 µL, 200 µL, 300 µL, 1000 µL).

2. Isihlungi vs Amathiphu Okungewona Okuhlunga

Amathiphu esihlungi:Amathiphu ahlungiwe afaka umgoqo omncane, ngokuvamile owenziwe nge-hydrophobic material, etholakala ngaphakathi kwethiphu. Lesi sihlungi sivimbela ukungcoliswa phakathi kwamasampuli kanye ne-pipette. Amathiphu okuhlunga ngokuvamile enzelwe ukusetshenziswa ezinhlotsheni ezithile zokuhlola. Isibonelo, uma isampula lonakalisayo, iguquguquka, noma i-viscous kakhulu ngokwemvelo, ingalimaza i-pipette. Ezimweni ezinjalo, amathiphu okuhlunga ayanconywa.

Ngaso sonke isikhathi lapho ufisa uketshezi, ama-aerosols akhiqizwa ngaphakathi kwephuzu le-pipette. Uma ungawasebenzisi amathiphu okuhlunga, lawa ma-aerosols kungenzeka angcolise i-pipette yakho namasampuli alandelayo, athinte imiphumela yakho yokuhlola. Ngakho-ke, amathiphu okuhlunga abiza kakhulu ekuhlolweni okunembayo.

Amathiphu angahlungi:Amathiphu angahlungi angamathiphu e-pipette asetshenziswa kakhulu ezindaweni zokucwaninga njengoba engabizi kakhulu kunamacebiso okuhlunga. Zifaneleka kakhulu kumasampula angenakukwazi ukungcola futhi akunakwenzeka ukulimaza i-pipette. njengokuhlukanisa i-plasmid DNA, nokulayisha amajeli e-agarose, phakathi kokunye. Kodwa-ke, abanazo izinzuzo zokuvimbela ukungcola zamathiphu okuhlunga, okuwenza angakufanelekeli ukuhlolwa okubucayi noma okubucayi.

3. Ukugcinwa Okuphansi vs Amathiphu Okugcina Okungaphansi (Okuvamile)

Amathiphu we-pipette okugcinwa okuphansiaklanyelwe ngokukhethekile ukunciphisa ukugcinwa koketshezi ngaphakathi kwethiphu, ukuqinisekisa ukudluliswa kwesampula okunembe kakhudlwana nangempumelelo. Lawa macebiso alungele ukusebenza ngoketshezi olune-viscous, olunamathelayo, noma oluyigugu lapho ukunciphisa ukulahleka kwesampula kubalulekile. Kodwa-ke, zibiza kakhulu kunamathiphu ajwayelekile, lezi zeluleko zilungele ukuqoqa amasampula ngesikhathi se-PCR, ukuhlanzwa kwamaprotheni, i-SDS-PAGE, i-cloning, i-DNA ne-RNA izicelo kanye nezicelo ezihlukahlukene zokuhlaziya amaprotheni.

4. Amathiphu amafushane vs. Ubude Obunwetshiwe

Amathiphu amafushane we-pipetteaklanyelwe ukusetshenziswa kumapuleti anemithombo eminingi, njengamafomethi angu-1536 noma angu-384, lapho usayizi wawo omncane usiza ukukhomba imithombo emincane ngokunembile. Lawa macebiso aphinde athuthukise i-ergonomics ngokuvumela i-pipetting eduze nebhentshi, ukunciphisa ubunzima bengalo ngesikhathi semisebenzi ephindaphindiwe. Ilungele ukuhlolwa komphumela ophezulu kanye nokuthuthukisa ukunethezeka kwaselabhorethri.

Amathiphu we-piette ubude obunwetshiwezinde kunamacebiso avamile, zinikeza ukulawula ukungcola okungcono ngokuvumela ukufinyelela phansi kwemikhumbi kuyilapho kunciphisa ukuthintana nesiqukathi. Lawa macebiso alungele ukusetshenziswa nemishini yelebhu njengamabhulokhi emithombo ejulile namashubhu e-microcentrifuge, okuqinisekisa ukuphathwa okunembile koketshezi ezindaweni okunzima ukufinyelela kuzo.

5. Amathiphu e-Pipette abanzi

Amathiphu we-pipette agcwele ububanziine-distal end ene-orifice efika ku-70% enkulu kunamathiphu ajwayelekile, isici siwusizo ikakhulukazi ekuqedeni ukugunda kwamaseli kanye nokumelana nokugeleza. Ukuwenza alungele ukuphatha amasampula okunzima ukuya kupayipi njengemigqa yeseli entekenteke, i-genomic DNA, i-hepatocytes, ama-hybridoma, nolunye uketshezi olubonakala kakhulu. Lawa macebiso anciphisa amandla okugunda emishini, avimbele ukuhlukana kwamaseli futhi aqinisekise ukusebenza kahle kwamaseli nokusebenza kahle kokucwenga.

6. I-Robotic Pipette Amathiphu

Amathiphu we-robotic pipettezenzelwe ukusetshenziswa nezinhlobonhlobo ze-automated liquid handling systems namarobhothi amapayipi. Lawa macebiso aqinisekisa ukuhambisana nemikhiqizo (Hamilton, Beckman, I-I-Agilent, I-I-Tecan, njll.) ku-automation yaselabhorethri, ithuthukisa ukunemba nokusebenza kahle ezinhlelweni zokusebenza eziphuma phambili. Amathiphu e-robotic alawulwa ngaphansi kokubekezelelana okuqinile uma kuqhathaniswa namathiphu we-pipette okwenziwa ngesandla. Lawa macebiso erobhothi ezenzakalelayo aqinisekisa ukunemba okuphezulu, ukunemba, kanye nokusebenza kahle ezinhlelweni zokusetshenziswa okuphezulu kuyo yonke imikhakha eyahlukene, okuhlanganisa i-genomics, i-proteomics, nocwaningo lwezemithi.

Isibonelo:

Amathiphu e-pipette e-conductiveangamathiphu akhethekile asetshenziswa ezinhlelweni zamapayipi ezizenzakalelayo eziklanyelwe ukunciphisa ukunqwabelana kwe-electrostatic charge ngesikhathi sokuphatha uketshezi. Lawa mathiphu abalulekile ezinhlelweni zokusebenza lapho ukuphazamiseka kwe-electrostatic kungase kuthinte ubuqotho besampula noma ukunemba kwamasistimu okubamba uketshezi oluzenzakalelayo.

7. Amathiphu akhethekile we-Pipette

Ezinye izinhlelo zokusebenza zidinga imiklamo yethiphu ye-pipette eyingqayizivele yemisebenzi ethile.

Izibonelo:

Amathiphu e-PCR:Amathiphu aklanyelwe ukusetshenziswa ezinqubweni ze-polymerase chain reaction (PCR) ukuvimbela ukungcoliswa okuvela ku-DNA ekhulisiwe.
Amathiphu e-Cryogenic:Idizayinelwe ngokukhethekile ukusetshenziswa namazinga okushisa aphansi kakhulu futhi ivamise ukuza esakhiweni esiqinile, esihlala isikhathi eside ukuze ibambe amasampula afriziwe.

Isiphetho

Ukukhethwa kwamathiphu we-pipette kuncike esimweni sokuhlolwa kanye nohlobo lwe-pipette olusetshenziswayo. Kungakhathaliseki ukuthi okokuphatha uketshezi oluvamile, ukuvimbela ukungcoliswa, noma ukusebenza ngamasampula athambile noma abizayo, ukuqonda izinhlobo nezici zamathiphu e-pipette kuqinisekisa ukudluliswa koketshezi okunembile nokusebenza kahle elabhorethri. Ngaso sonke isikhathi khetha ithiphu le-pipette elifanele lezidingo zakho zocwaningo ukuze uqinisekise imiphumela emihle nokwethembeka.