Isingeniso sika-Elisa Plate

ELISA PLATE: Ku-Enzyme Linked Immunosorbent Assay (ELISA), ukuhlanzeka, ukugxila kanye nesilinganiso sama-antigens, amasosha omzimba, amasosha omzimba anelebula noma ama-antigen ahilelekile ekuphenduleni kwe-immunological; uhlobo lwe-buffer, ukugxila kanye Nezimo ezifana namandla e-ionic, inani le-pH, izinga lokushisa lokusabela nesikhathi kudlala indima ebalulekile. Ngaphezu kwalokho, ingaphezulu le-polystyrene yesigaba esiqinile (i-Polystyrene) njengesithwali nayo idlala indima ebalulekile ekukhangiseni ama-antigen, amasosha omzimba noma ama-antigen-antibody complexes.

Ama-antigen, amasosha omzimba kanye namanye ama-biomolecule akhangiswa endaweni ephathwayo ngokusebenzisa izindlela ezihlukahlukene, okuhlanganisa i-adsorption passive ngokusebenzisa amabhondi e-hydrophobic, amabhondi e-hydrophobic/ionic, isibopho esihlangene ngokwethulwa kwamanye amaqembu asebenzayo njengamaqembu e-amino nekhabhoni, nangokuguqulwa kwendawo. . Ukuhlanganiswa kwe-Hydrophilic ngemva kocansi.

IElisa Platezingahlukaniswa zibe 48-umthombo kanye 96-kahle ngokwenani lezimbobo. Esetshenziswa kakhulu yi-96-well, okufanele ikhethwe ngokuya ngomfundi wakho we-microplate.

Ngaphezu kwalokho, kukhona okungahlukaniseki futhi okungahlukaniseki. Kulabo abangadonsi, ama-slats ebhodini lonke axhunywe ndawonye. Khona-ke, kulabo abathintekayo, ama-slats ebhodini ahlukaniswa, futhi amabhodi ahlukanisiwe Kukhona imichilo engu-12-hole kanye ne-8-hole. Ngokuvamile, amapuleti anamalebula e-enzyme akhiphekayo asetshenziswa kakhulu namuhla. Uma uke wawathenga ngaphambili amapuleti anjalo, ungavele uthenge amapheshana anelebuli ye-enzyme manje.

Nakuba ama-microplates enziwe abakhiqizi abahlukene abukeka afanayo jikelele, eminye imininingwane emincane izohluka, njengesakhiwo, njll. Lokhu ikakhulukazi ngoba adinga ukusetshenziswa nabafundi be-microplate abahlukene. Ngakho-ke, uma usebenzisa Lapho ukhetha ukuthenga isifundi se-microplate, kufanele futhi ucabangele ukuthi umfundi wakho we-microplate ubukeka kanjani. Kodwa ngokuvamile zishintshwa, ezinye zizohluka. Ngoba impahla yepuleti le-enzyme ngokuvamile i-polystyrene (PS), futhi i-polystyrene ayinakho ukuzinza kwamakhemikhali futhi ingancibilika yizinhlobonhlobo zezincibilikizi eziphilayo (njengama-hydrocarbon anephunga elimnandi, ama-halogenated hydrocarbons, njll.), futhi azogqwaliswa ama-asidi aqinile. kanye nama-alkali. , ayimelani namafutha, futhi kulula ukushintsha umbala ngemva kokuchayeka ekukhanyeni kwe-ultraviolet, ngakho qiniseka ukuthi uyazinaka lezi uma usebenzisaElisa Plate.