Intambo yangaphakathi noma intambo yangaphandle, ungakhetha kanjani izitsha ze-cryogenic?

Ocwaningweni locwaningo lwesayensi, ama-cryovials ayithuluzi elibalulekile lokugcina amaseli isikhathi eside, amagciwane, amasampula ebhayoloji, njll., ehlinzeka ngendawo yokugcina ezinzile, enezinga lokushisa eliphansi lamasampula ebhayoloji ukuze kuqinisekiswe umsebenzi nobuqotho bamasampuli.

Kodwa-ke, lapho sikhipha amasampula agcinwe isikhathi eside esiqandisini sokushisa okuphansi kakhulu noma ithangi le-nitrogen eliwuketshezi, ngokuvamile sithuswa ngokuzumayo umsindo oklabileyo weshubhu le-cryogenic futhi sibanjwe inhliziyo. Ukuqhuma kwamashubhu e-cryovials ngeke nje kubangele ukulahlekelwa amasampula okuhlola, kodwa kungase futhi kubangele ukulimala kubasebenzi abahlolwayo.

Yini edala ukuthi kuqhume Isitsha Sokugcina? Sikuvimbela kanjani lokhu ukuthi kungenzeki?

Imbangela eyinhloko yokuqhuma kweshubhu lesiqandisi izinsalela zenitrogen ewuketshezi ngenxa yokucinana komoya.Lapho ishubhu lesampula le-cryopreservation likhishwa ethangini le-nitrogen eliwuketshezi, izinga lokushisa ngaphakathi kweshubhu liyakhuphuka, futhi i-nitrogen enguketshezi eshubhu ihwamuka ngokushesha futhi ishintshe. kusukela oketshezini kuya kugesi. Ngalesi sikhathi, ithubhu ye-cryovials ayikwazi ukususa i-nitrogen eyeqile ngesikhathi, futhi iqoqana eshubhu. Umfutho we-nitrogen ukhuphuka kakhulu. Lapho umzimba we-tube awukwazi ukumelana nokucindezela okuphezulu okukhiqizwa ngaphakathi, uzoqhuma, kubangele ukuqhuma kwepayipi.

Ngaphakathi noma ngaphandle?

Ngokuvamile singakhetha ishubhu le-cryovial elijikelezayo elinomoya omuhle. Ngokuya ngesakhiwo sekhava yeshubhu kanye nomzimba weshubhu, lapho i-nitrogen ewuketshezi kushubhu ye-cryovial ejikeleza ngaphakathi ihwamuka, kulula ukuyikhipha kuneshubhu le-cryovial elijikeleza ngaphandle. Ngaphezu kwalokho, umehluko wokuklama wekhwalithi efanayo yamashubhu e-cryogenic uzobangela ukuthi ishubhu ye-cryopreservation ejikeleza ngaphakathi ihwamuke. Ukusebenza kokuvala kwepayipi elifakiwe kungcono kunokwepayipi eliboshwe ngaphandle, ngakho-ke mancane amathuba okuba kubangele ukuqhuma kwepayipi.

Isigqoko sangaphandle siklanyelwe ukuqandisa ngomshini, okwenza singafinyeleleki kancane kusampula ngaphakathi kweshubhu futhi ngaleyo ndlela kuncishiswe amathuba okungcoliswa kwesampula. Ingafakwa ngqo esiqandisini ukuze iqandiswe, futhi ayifanele ukugcinwa kwenitrogen ewuketshezi.

I-I-Cotaus cryovials tubengamakhodi amathathu:

I-1.I-tube cap kanye nomzimba wepayipi kukhiqizwa kusukela ku-batch efanayo kanye nemodeli ye-PP yezinto zokusetshenziswa, ngakho-ke i-coefficient yokwandisa efanayo iqinisekisa ukuvala kunoma yikuphi ukushisa. Ingakwazi ukumelana nezinga lokushisa elingu-121℃ kanye nokuvalwa kwe-high pressure futhi ingagcinwa endaweni engu -196℃ eyi-nitrogen eyi-liquid.

2. I-cryo tube ejikelezayo iklanyelwe amasampula aqandayo. I-screw cap ezungezisayo ngaphandle inganciphisa ithuba lokungcola lapho ubamba amasampuli.

3. Internally rotating cryovials are designed for freezing samples in the liquid nitrogen gas phase. The silicone gasket at the mouth of the tube enhances the sealing of the cryovial.

4. Umzimba weshubhu unokukhanyela okuphezulu futhi udonga lwangaphakathi lwenzelwe ukuthelwa kalula koketshezi futhi akukho nsalela ekuthathweni kwesampula.

5. I-2ml Ishubhu ye-Cryovial iguqulelwe ku-rack yepuleti ye-SBS evamile, futhi i-tube cap ezenzakalelayo ingashintshwa ukuze ivumelane nesiteshi esisodwa kanye nezivuli ze-cap ezizenzakalelayo zeziteshi eziningi.

6. Indawo yokumaka emhlophe nesikali esicacile sikwenza kube lula kubasebenzisi ukumaka nokulinganisa umthamo. Inhlanganisela yekhodi ye-QR engezansi, ibhakhodi eseceleni, nekhodi yedijithali yenza ulwazi lwesampula lucace lapho uthi nhlá, kunciphisa kakhulu ubungozi bokudideka noma ukulahleka kwesampula.

Izitsha ze-Cotaus ezintathu-in-one ze-cryogenic ekuqaleni zikhiqizwa kwi-polypropylene yezinga lezokwelapha. Amandla amanje angu-1.0ml no-2.0ml, kanti okunye ukucaciswa kungenziwa ngokwezifiso ngokuya ngezidingo zamakhasimende. Ngokusebenza kwayo okuhle kakhulu nokuklama okulula, inikeza ukukhetha okungcono kubacwaningi besayensi. Noma ngabe ingaphakathi noma ingaphandle, ingahlangabezana nezidingo zakho ezihlukene zokuhlola futhi yenze indlela yakho yocwaningo lwesayensi ibe bushelelezi. Khetha i-Cotaus, yenza imiphumela yakho yokuhlola ivelele kakhulu!