Ukufika Okusha | IYADAYISWA | Black Elisa Plates

Ezimweni ezihlukahlukene kusayensi yempilo, ukuzimisela okufika ngesikhathi, okusebenzayo kanye nokonga kanye nomthamo wama-antigen noma amasosha omzimba akhona kusampula kuyisici esibalulekile.

I-Enzyme-linked immunosorbent assay (ELISA) ibonakale iyithuluzi elibalulekile locwaningo nelokuxilonga lokulinganisa amasosha omzimba noma ama-antigen kumasampula ebhayoloji ngokufakwa kwama-antigen aziwayo noma amasosha omzimba ebusweni besiphathi sesigaba esiqinile, esivumela i-enzyme ( ikakhulukazi i-HRP) -okubhalwe ukuthi i-antigen-antibody reactions endaweni yesigaba esiqinile. Le nqubo ingasetshenziswa ukuthola ama-antigen e-molecule amakhulu kanye namasosha omzimba athile, njll. Inezinzuzo zokushesha, ukuzwela, ukulula, futhi umthwali kulula ukumisa. Nokho, ukuzwela nobubanzi obuguquguqukayo bokutholwa kwe-ELISA bukhawulelwe kakhulu ukushiyeka kwendlela yokumunca ukukhanya ngenxa yethonya elikhulu lezimo zangaphandle ekushintsheni kombala wesixazululo kanye nobubanzi bomugqa obusebenzayo obuphansi bevelu ye-OD.

Ubuchwepheshe be-DELFIA ---- bumane buwukushintsha i-enzyme ye-HRP esikhundleni se-enzyme ye-lanthanide chelate (Eu, Sm, Tb, Dy) elebula kuma-antibody okutholayo emivivinyweni evamile ye-ELISA. Ama-lanthanides asetshenziswa ku-DELFIA ikilasi elikhethekile lama-elementi e-fluorescent, abeka izidingo ezintweni zokuhlola --- amapuleti ka-Elisa. I-Lanthanides inezikhathi zokuphila ze-fluorescence zama-microseconds noma ama-milliseconds, okuthi uma kuhlanganiswe nokutholwa okuxazululwe isikhathi kunciphisa kakhulu ukuphazamiseka kwengemuva le-autofluorescence, futhi ukushintshwa kwe-Strokes's okubanzi kuthuthukisa kakhulu ukuzwela kokuhlolwa.

Iningi le-ELISA likhetha ipuleti lokulebula le-enzyme esobala njengesithwali nesitsha, kepha ukukhanya okukhishwa ekuphenduleni kwe-luminescence kuyi-isotropic, ukukhanya ngeke kuhlakazeke nje ukusuka endaweni eqondile, kodwa futhi kuzohlakazwa ukusuka endaweni evundlile, futhi kuzohlakazeka. ukudlula kalula igebe phakathi kwezimbobo ezihlukahlukene zecwecwe lokulebula le-enzyme esobala kanye nodonga lwezimbobo.Izimbobo ezingomakhelwane ziyahlangana futhi zithinte imiphumela yokuhlola.

Amapuleti e-Elisa amhlophe angasetshenziselwa ukuthola ukukhanya okubuthakathaka futhi ajwayele ukusetshenziselwa ukwakhiwa kombala we-chemiluminescence kanye ne-substrate (isb. ukuhlaziywa kofuzo lwentatheli ye-luciferase ekabili).

I-Black White Elisa Plates inesiginali ebuthakathaka kunamapuleti amalebula e-enzyme emhlophe ngenxa yokumunca kwawo ukukhanya, futhi ngokuvamile asetshenziselwa ukuthola ukukhanya okunamandla, njengokutholwa kwe-fluorescence.

Izinzuzo ze-Cotaus®Elisa Plates

● Ukubopha okuphezulu

Amapuleti e-Cotaus®Elisa aneshubhu elimnyama enziwe ngezinto ezingezona ezizikhanyelayo, indawo engaphezulu yelashwe ukuze ithuthukise kakhulu amandla ayo okubopha amaprotheni, angafinyelela ku-500ng IgG/cm2, kanti isisindo samangqamuzana amaprotheni abophekile angaphezulu kuka-10kD. .

● I-fluorescence yangemuva ephansi iqeda izinkinga ezibangelwa ukusabela okungaqondile.

amabhavu amnyama angaqeda ukuphazanyiswa kwe-fluorescence yangemuva ebuthakathaka ngoba izoba nokumuncwa kwayo kokukhanya.

● Idizayini ekhiphekayo

Idizayini ehlukanisekayo yefreyimu yepuleti le-enzyme emhlophe nama-slats e-enzyme emnyama kulula kakhulu ukusebenza. Naka isenzo sokuhlakaza, ungaphoqi ukuphuka ekugcineni, kungenjalo kuyoba lula ukuphuka.

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