Kungani izitsha zesiko lamaseli zidinga ukwelashwa okungaphezulu?

Ukuze kuhlangatshezwane nezidingo zokuhlolwa kwe-cell culture kanye nokuzuza imiphumela efunekayo, ngokuvamile sisebenzisa ukwelashwa kwe-TC, ukwelashwa okuthuthukisiwe kwe-TC kanye nokwelashwa okunamathiselwe okuphansi kakhulu kwamaseli amisiwe.

1. Ukwelashwa kwe-TC , kulungele isiko lamaseli anamathelayo

Ngokwelashwa okukhethekile kwe-vacuum gas plasma, ungqimba olungaphezulu lungakhokhiswa ngokungaguquki futhi ngokulinganayo kuwo womabili amaqembu amahle namabi isikhathi eside, okuqinisekisa ukunamathiselwa kweseli okufana kakhulu futhi okuzinzile. Ukwethulwa kokushaja okuphindwe kabili kwenza indawo ye-TC ye-endothelial, i-hepatocyte ne-neuron cell culture ibe nokunamathela okungcono nokusabalalisa kunezindawo ezifanayo ze-TC, futhi ingakwazi ukufeza ukusebenza kahle kokunamathela kwamangqamuzana ukuhlangabezana nesiko lezinga eliphakeme lamaseli anamathelayo. Indawo engaphezulu ingafinyelela ukusebenza okuhle kakhulu kokunamathela kweseli futhi ihlangabezane nezinga eliphezulu lesiko leseli elinamathelayo.

2. Ukwelashwa okuthuthukisiwe kwe-TC, okulungele Amasiko Eseli Nezimfuneko Eziphakeme Zokunamathela

Ukwelashwa okuthuthukisiwe kwesiko lezicubu, uma kuqhathaniswa nemikhiqizo ejwayelekile ephathwe yi-TC, indawo ethuthukisiwe ye-TC inamandla okukhuthaza ukunamathela kwamaseli nokwandiswa, ukwanda okusheshayo kwamangqamuzana, ingasetshenziselwa ukusabalalisa amaseli afunayo, njengamaseli ayinhloko noma azwelayo, kanye namaseli akhuliswe ngaphansi kwezimo ezivimbela ukukhula (i-serum engenayo i-serum noma encishisiwe), ukwanda okusheshayo kwamangqamuzana, ukukhuthazwa kokunamathela kwamangqamuzana nokwandiswa, kungagcinwa ekamelweni lokushisa ukuze kusetshenziswe izinhlelo zokusebenza eziningi.

3. Uchungechunge lwe-adsorption oluphansi kakhulu lwesiko lamaseli amisiwe

I-amphoteric molecular polymer ekhethekile imbozwe phezu komkhumbi wamasiko. Njengoba le nhlanganisela i-hydrophilic ikakhulukazi, ama-amphoteric molecule angakhanga ama-molecule amanzi ukuze akhe udonga lwamanzi, ukuze amangqamuzana, ama-molecule amaprotheni, ama-bacterium nezinye izinto zingakwazi ukunamathela emkhunjini we-culture, okuholela ekubambeleleni kwamangqamuzana aphansi kakhulu. ingakhuliswa ngokumiswa isikhathi esingaphezu kwezinsuku eziyi-15.

Ingasetshenziselwa isiko lamaseli e-fetus, ama-hemocyte namanye amaseli adinga ukukhula endaweni yesiko lokumiswa, kanye nokukhuthaza isiko lamaseli e-spheroid e-3D nama-organoids, futhi inezici zokulwa nokunamathela kumaseli aqinile anamathelayo.

I-Cotaus inikeza uhla olugcwele lwemikhiqizo yesiko lamaseli, ukuze uthole imininingwane eyengeziwe yomkhiqizo, sicela usithinte.