Izicelo Ezivamile ze-ELISA Plates

Njengethuluzi lokuhlola, isakhiwo esiyinhloko se-Ipuleti le-ELISAuchungechunge lwama-microplates aqukethe izinto zesigaba esiqinile (njengamaprotheni namasosha omzimba). Ekusetshenzisweni kwepuleti le-ELISA, isampula elizohlolwa lizosabela nge-molecule ethile enelebula le-enzyme, bese kukhiqizwa ukuguqulwa kombala okubonakalayo ngokungeza i-matrix substrate, futhi okuqukethwe noma umsebenzi we-molecule eqondiwe izobalwa. noma ihlolwe ngokuthola isignali yokumunca noma ye-fluorescence. Okulandelayo ukusetshenziswa okuvamile kwamapuleti e-ELISA emikhakheni ehlukene:

1. Ukuhlaziywa kobuningi bamaprotheni: Amapuleti e-ELISA angasetshenziswa ukukala ukugxila nokusebenza kwamaprotheni kumasampula ebhayoloji njenge-serum nama-cell supernatants, ukuhlinzeka ngamathuluzi anamandla okuthola izimpawu zesimila, amasosha omzimba egciwane le-hepatitis, izimpawu zokulimala kwe-myocardial, njll., kanye nokusiza odokotela ekuxilongeni kusenesikhathi nasekuhloleni izifo.

2. Ukuqapha i-Cytokine: Ocwaningweni lwe-immunology,ELISA amapuletiingakwazi ukukala amazinga e-cytokine kuma-cell culture supernatants noma uketshezi lwezicubu, okusiza ukuqonda izinqubo zebhayoloji ezifana nezimpendulo zokuzivikela komzimba nezimpendulo zokuvuvukala, futhi kubaluleke kakhulu ekuthuthukisweni kokwelashwa okusha nezidakamizwa.

3. Ucwaningo lwe-Nucleic acid: Ngokusebenzisa amapuleti e-ELISA, ososayensi bangathola futhi bahlaziye okuqukethwe kanye nomsebenzi we-DNA noma i-RNA, banikeze ukusekelwa kwedatha yocwaningo lwebhayoloji yamangqamuzana njengokusho kwezakhi zofuzo nokulawulwa kofuzo, futhi baqhubeke bakhuthaze ukuthuthukiswa kwezinkambu ezifana nokwelashwa kwezakhi zofuzo. kanye nokuhlelwa kofuzo.

4. Ucwaningo lomsebenzi we-enzyme: Amapuleti e-ELISA angakwazi ukukala ngokunembile umsebenzi we-enzyme, asize abacwaningi baqonde umsebenzi kanye nendlela yokulawula yama-enzyme ezintweni eziphilayo, futhi banikeze izinkomba ezibalulekile zocwaningo kubunjiniyela be-enzyme, ubunjiniyela be-metabolic neminye imikhakha.

5. Ucwaningo lokusebenzisana kwamangqamuzana:ELISA amapuletiingasetshenziswa hhayi kuphela ukukala okuqukethwe kwama-molecule, kodwa futhi ukutadisha ukusebenzisana phakathi kwama-molecule. Ngokuhlanganisa ubuchwepheshe obufana ne-surface plasmon resonance kanye ne-fluorescence resonance energy transfer, inqubo yokubopha nokuhlukanisa phakathi kwama-molecule ingabhekwa ngesikhathi sangempela, inikeze imibono emisha nezindlela zokuklama izidakamizwa, ukusebenzisana kwamaprotheni kanye nolunye ucwaningo.