Kungani izinto ezisetshenziswayo ze-PCR ngokuvamile zenziwe nge-PP?

"Njengoba sazi sonke, i-PCR iyindlela yokuhlola eyisisekelo kumalabhorethri we-biochemical." Imiphumela yokuhlolwa ihlale ingagculisi, okungenzeka kungenxa yokungcoliswa okuncane kwe-PCR plastic consumables, noma ukuphazamiseka kokuhlolwa okubangelwa ukwethulwa kwama-inhibitors. Kunesinye isizathu esibaluleke kakhulu: Ukukhetha okungafanele kwezinto ezisetshenziswayo nakho kuzoba nomthelela omkhulu emiphumeleni yokuhlola.

Kunezizathu eziningi ezithinta imiphumela yokuhlolwa kwe-PCR: ngokuvamile kunezinhlobo ezingu-7 ezilandelayo.

1. Iziqalo: Iziqalo ziwukhiye wokusabela okuthile kwe-PCR, futhi ukucaciswa kwemikhiqizo ye-PCR kuncike ezingeni lokuhambisana phakathi kwama-primer kanye ne-DNA yesifanekiso;

2. I-Enzyme nokugxila kwayo;

3. Izinga nokugxila kwe-dNTP;

4. Isifanekiso (i-target gene) i-nucleic acid;

5. Ukugxila kwe-Mg2+;

6. Ukusetha izinga lokushisa nesikhathi;

7. Inani lemijikelezo;

8. Izimpahla, izinto ezisetshenziswayo, njll.

Phakathi kwezinto eziningi ezinomthelela, izinto ezisetshenziswayo zingenye yezinto ezibaluleke kakhulu nezinganakwa kalula.

Kunezinhlobo eziningi zeIzinto ezisetshenziswayo ze-PCR: Amashubhu angu-8, amashubhu anevolumu ephansi, amashubhu ajwayelekile, angagqoke iziketi, onesiketi esiphakathi, onesiketi esigcwele, kanye nochungechunge lwamapuleti e-PCR kanye ne-qPCR. Kunzima kakhulu ukukhetha, futhi kunezinkinga eziningi Ezijwayelekile, ake sibheke izinkinga ezikhethwa yiwo wonke umuntuIzinto ezisetshenziswayo ze-PCR, futhi kanjani ukuzixazulula?

KunganiIzinto ezisetshenziswayo ze-PCRngokuvamile eyenziwe PP?

Impendulo: Izinto ezisetshenziswayo ze-PCR/qPCR ngokuvamile zenziwe nge-polypropylene (PP), ngenxa yokuthi iyinto engasebenzi ngokwezinto eziphilayo, indawo engaphezulu akulula ukunamathela kuma-biomolecules, futhi inokumelana okuhle kwamakhemikhali nokubekezelelana kwezinga lokushisa (ingenziwa i-autoclaved ku-121 degrees) amagciwane. futhi ingamelana nokushintsha kwezinga lokushisa ngesikhathi sokuhamba ngebhayisikili okushisayo). Lezi zinto ngokuvamile zithintana ngokuqondile nama-reagents noma amasampula, ngakho-ke izinto ezisezingeni eliphezulu kanye namasu okucubungula amahle adinga ukukhethwa ngesikhathi sokukhiqiza nokulungiselela.