Ingabe abangani elabhorethri bavame ukudidwa umehluko phakathi kwamashubhu e-PCR, amashubhu e-EP, namashubhu angamashubhu ayisishiyagalombili? Namuhla ngizokwethula umehluko nezici zalaba abathathu

Ingabe abangane elabhorethri ngokuvamile ukudideka umehluko phakathiI-PCR tubes, amashubhu e-EP, namashubhu angamashubhu ayisishiyagalombili? Namuhla ngizokwethula umehluko nezici zalaba abathathu

1. I-PCR tube

I-PCR tubes ajwayele ukusetshenziswa ekuhloleni kwebhayoloji. Isibonelo, amashubhu e-Cotaus®PCR asetshenziselwa ikakhulukazi ukuhlinzeka ngeziqukathi zokuhlolwa kwe-PCR (polymerase chain reaction), ezingasetshenziswa ekuguquleni izakhi, ukulandelana, i-methylation, i-molecular cloning, i-gene expression, i-Genotyping, imithi, isayensi ye-forensic neminye imikhakha. Ishubhu le-PCR elivamile lakhiwa umzimba weshubhu kanye nesembozo, futhi umzimba weshubhu nekhava kuxhunywe ndawonye.

Ithuluzi le-PCR lakudala lalingenayo ikhava eshisayo. Ngesikhathi senqubo ye-PCR, uketshezi olungaphansi kweshubhu lungahwamuka luye phezulu. Isembozo esiyi-convex (okungukuthi, isiqongo esiyindilinga) saklanywa ukwenza lula ukuhwamuka koketshezi ukuze kufinyele futhi kugeleze phansi. Nokho, ithuluzi lamanje le-PCR ngokuyisisekelo liwuhlobo lwekhava eshisayo. Izinga lokushisa phezulu kwekhava ye-PCR liphezulu futhi izinga lokushisa ngaphansi liphansi. Uketshezi olungezansi akulula ukuhwamuka luye phezulu, ngakho-ke iningi labo lisebenzisa izembozo eziyisicaba.

2. EP tube

Ngenxa yokuthi ithubhu ye-centrifuge yaqala ukusungulwa futhi yakhiqizwa ngu-Eppendorf, ibizwa nangokuthi i-EP tube.

Umehluko omkhulu phakathiI-PCR tubes kanye namashubhu e-microcentrifuge ukuthi amashubhu e-microcentrifuge ngokuvamile anezindonga zamashubhu aminyene ukuqinisekisa izidingo ze-centrifugation, kuyilaphoI-PCR tubes anezindonga zamashubhu ezacile ukuze kuqinisekiswe isivinini sokudlulisa ukushisa nokufana. Ngakho-ke, lezi ezimbili azikwazi ukuxutshwa ekusebenzeni okusebenzayo, ngoba amashubhu e-PCR amancane angase aqhume ngenxa yokungakwazi ukumelana namandla amakhulu e-centrifugal; ngokufanayo, amashubhu e-microcentrifuge aminyene azothinta umphumela we-PCR ngenxa yokudlulisa ukushisa kancane nokudluliswa kokushisa okungalingani.

3.amashubhu ayisishiyagalombili

Ngenxa yomthwalo osindayo wokuhlolwa kwenqwaba kanye nokusebenza okungalungile kweshubhu eyodwa, kwasungulwa amashubhu ayisishiyagalombili emigqeni.I-I-Cotaus®I-PCR 8-strip tube yenziwe nge-polypropylene engenisiwe, futhi isembozo seshubhu sifaniswa nomzimba weshubhu, onokusebenza okuhle kakhulu kokuvala. Ngesikhathi esifanayo, inokuvumelana nezimo okuqinile futhi ingahlangabezana nezinjongo ezihlukene zokuhlola.